Matching Pair of Human-Fit NA Novelty Emerges Independently in Two Distinct Locales during August 2009

The University of Padova deposited 23 Neuraminidase segments at GenBank yesterday. One particularly merits discussion. We were keyed to the sequence by the rare 106I and 248N human pairing, but even more human fitness acquisition was at work upon review.  The NA of A/Italy/180, sampled in August 2009 from a 47M, brings another unique face into ΣPF11.  

NA Amino Acid Codings to 1 Novel Polymorphism and 2 Independent Co-Emergent Signals

•    45K
•  386D
•  426L (synonymous C1276T)

45K is novel to ΣPF11 and is rare to the sub-type being found in only 3 previous H1N1 human sequences. 386D and the C1276T SNP coding for a synonymous 426L are found twice in ΣPF11 and emerged paired and coincident with the Italy180 sequence in the secondary and distinct locale of Catalonia, Spain on a different background during the same time period. The 386D and syn426L polymorphisms appear to be human-fit as they are represented in all recent human H1N1 Seasons (2005-2009).

Neuraminidase Triple Combination
106I, 248N, 286S

Only 25 sequences currently match the 146 amino acids from 106I to 248N, including the Italy134 sequence recently profiled demonstrating the NA Triple Combination herald for Pandemic 2.0.

This new Italian sequence is a marvel in itself, but the argument against random mutation gains strength upon a similar and apparently independent co-emergence. Two perfectly novel introductions paired onto two divergent backgrounds in two different geographies suggest a randomness redundancy. 

A/Catalonia/S1276, sampled 2009-08-19 from a 13F, also demonstrates two of the three novel introductions, but onto a separate NA background.  CatS1276 is a perfect nucleotide match to Italy180 except at 742G generating 248D (1216 of 1217 aligned residues).  Like many of the Catalonia sequences, the initial 47 amino acids of CatS1276 are absent or we might find the 45K there as well.  Even without the full sequence, CatS1276 is unique and stand-alone within ΣPF11 due to the swine 248D conjoined with 386D and C1276T.

45K
Novel to ΣPF11.
Progenitors may include:
A/Washington/03/2009 H1N1 with HA 230I & NA 386D, syn426L
A/New Jersey/30/2008 H1N1 with HA 230I & NA 386D, syn426L
A/Thailand/271/2005    H1N1 with HA 225G, 261N, 263D, 270T & NA:45K, syn346V

386D
CatS1276 is the singular ΣPF11 peer.
Progenitors may include:
Seasonal H1N1 2005, 2006, 2007, 2008 and 2009 (Consensus)
Avian H1N1

syn426L
CatS1276 is the singular ΣPF11 peer.
Seasonal H1N1 2005, 2006, 2007, 2008 and 2009 (Extensive)
Swine H1N1 1990-1999
WSN33

Paired genetic introductions onto disparate backgrounds are not a spline for randomness.  Perhaps a less random examination of the database at hand would provide additional observational evidence for a new school of logic?  Without question, human-fit genetics from previous Seasonal H1N1 and H3N2 are being gained by the Hydra pandemic strains.  In this case, the sub-segment acquisitions are precise and clear.

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