Cameroon 230I Permissive Sequences Carry H3N8 and H7N7 Zoonotic Matches

Recently published sequences from Cameroon show homology to backgrounds that have supported HA 230I.  Several of these backgrounds carry homology to animal reservoirs and Cameroon follows suit.  These two young boys, ages 10 and 7, who were part of a transmission chain extended over 13 days may or may not have had close contact with animals. Cameroon10v_00812_7M_2010_03_23 is an exact HA match with Cameroon10v_00751_10M_2010_03_10.

The sequences are potentially related to the severe wave in the UK that has filled hospitals and, more importantly, Intensive Care Units to capacity placing many large medical trusts into "black" alert.  English elective surgeries are actively being cancelled.  Families are being told not to attempt a hospital visit in the case of Influenza due to the infrastructure strain.  Emergency departments are forced to place patients on gurneys due to no beds being available while operating theatres and lounges are being hastily converted to patient rooms.

Animal influences on the genetics of human-infecting Influenza like the pH1N1 strain may generate extreme outcomes and variant clinical behaviour. In these cases from Cameroon, the syn350G, 457R, syn461K and syn466G, among others, share homology with animal reservoirs as noted on the sequence details. We have discussed widely the HA 188T found in the emerging sub-clade including Australia, the UK and Iran. HA 188T is also found in the H7N7 reservoir, as is the syn350G on these Cameroon sequences.

The 457R and syn466G appear together within the H3N8 reservoir and are paired on a particularly interesting set of related horse and dog sequences from Australia.  These two changes fall into a domain band that is related to the emerging sub-clade from Australia to the UK.

• canineSydney6525_2007_10_14
• canineSydney6692_2007_10_15
• equineSydney6085_2007_10_10

GeneWurx_UK_December_Emerging_Genetics_v3.xls

. . . . Cameroon10v_00812_7M_2010_03_23 (
. . . . . . . . 100N [UKWhiteChapel4780352_2010_10_26
. . . . . . . . . . . . . . . . with 128D, syn131S, syn210S, 377K],
. . . . . . . . syn137A [UKCambridge118_2010_11_19
. . . . . . . . . . . . . . . . . . . . with syn363G, syn455Q, syn474C,
. . . . . . . . . . . . . . . . NorthCarolina56_2009_12_07
. . . . . . . . . . . . . . . . . . . . with 100N, 377K],
. . . . . . . . 218V [Minnesota04_18M_2010_03_22
. . . . . . . . . . . . . . . . with 165N, 230I, syn346G,
. . . . . . . . . . . . . Pennsylvania14_20M_2009_06_07
. . . . . . . . . . . . . . . . with 158E, 523I],
. . . . . . . . syn270I,
. . . . . . . . syn343G,
. . . . . . . . syn350G [H7N3, H7N7],
. . . . . . . . . . . [IranShiraz1_2010_02
. . . . . . . . . . . . . . . with 218A, 228R,
. . . . . . . . . . . swThaiCURA4_2009_11
. . . . . . . . . . . . . . . with 226R, syn484N],
. . . . . . . . 377K,
. . . . . . . . 457R [H2N3, H3N8, H4, H5N1, H7N3],
. . . . . . . . . . . . . [swJapanOkinawa2_2005
. . . . . . . . . . . . . . . . . . . with 225G],
. . . . . . . . syn461K [H2N3, H5N1 Human, Civet & Avian, H6N1, H11],
. . . . . . . . . . . . . . .  [Iran16273_2009_11_22
. . . . . . . . . . . . . . . . . . . . with syn55L, 226R],
. . . . . . . . syn466G [H2N3, H3N8, H4N8, H6N1, H7N3, H9N2, H10N7],
. . . . . . . . . . . . . . . [cheetahCA30954_2009_11_17
. . . . . . . . . . . . . . . . . . . . with syn169I, 200S])

. . . . Cameroon10v_00751_10M_2010_03_10 (
. . . . . . . . 100N,
. . . . . . . . syn137A,
. . . . . . . . 218V,
. . . . . . . . syn270I,
. . . . . . . . syn343G,
. . . . . . . . syn350G,
. . . . . . . . 377K,
. . . . . . . . 457R,
. . . . . . . . syn461K,
. . . . . . . . syn466G)

Please visit GeneWurx.com for insight into the latest published studies.