HA 230I RBS Polymorphism Potential for Europe

Current trending indicates a reportable potential for the M230I polymorphism spreading on the Hemagglutinin of PF11. This Receptor Binding Domain change may enhance Vaccine Escape from the currently selected vaccine target candidate, CA/07 X181. 

For those who are following the prediction on February 25, 2010, the pandemic reservoir now shows multiple instances of multiple encodings for 230I.  Model adjustment and data transparency allowed a second set of detailed geographic predictions on 2010-08-09 that have also found traction.

The 230I bearing sequences meeting the prediction are documented in the detailed discussion on Vaccine Escape that demonstrates a 100% change rate in the pandemic influenza (pH1N1) reservoir at the critical HA genetics range between amino acid positions 186 and 248.  89% of the amino acid positions have notated revisions.

Expectations for the M230I polymorphism, that first came to our notice for zoonotic concern on the H5N1 human fatality cluster, have now been revised based on the most current public data.  The GeneWurx model approximates that HA 230I will appear in the PF11 RBS according to the following geographic probabilities.

• 75% probability in Germany sampled by 2010-12-01.
• 75% probability in Spain sampled by 2010-12-01.
• 85% probability sampled by 2010-12-01 in one of:
• Denmark
• Norway
• Sweden
• 5% or less probability of HA 230I within 60 days of conserving across PF11.
• 7% probability of HA 230I within 180 days of conserving on one or more Hydrae.

This HA polymorphism guided an investigation suggesting that  anti-viral resistance from the NA H275Y revision is not the only genetic concern with over-usage.  Anti-viral drug usage may drive 230I genetic acquisition, with a higher potential among children and young adults (0-24 years old). The sparsity of complete sequences, discounted by the lack of required meta-data, allows only a rough postulation on the mechanism.

An accelerated viral self-revision, subsequently moving toward M230I, may be modulated by the substantially higher rates of host exposure in the lower age brackets. Additionally, that group’s multiplicity of exposure to a wider variation of influenza strains may factor into the host response/viral revision equation. In plain terms, the viral reservoir is being forced toward hard labour in order to infect and re-infect the youth.

Just as hard work builds muscle, viral exertion against a complex host response challenges the reservoir subset to revise toward an optimal solution. 230I is an excellent and proven tertiary response for a virus on this type of offensive.

These probabilities will be updated as additional data is made public.  Transparency at this post-pandemic stage is essential to formulate viable responses for the risk groups.  Release of sequences and clinical data of a finer detail and higher quantity will allow information-based decisions.

Please visit GeneWurx.com for insight into the latest published studies.

HA 230I Documented in Texas with 225E RBD Revision

The United States Air Force School of Aerospace Medicine published a sequence on 2010-08-20 with multiple RBD changes sampled on 2009-10-04 from an 18 year old male in Texas.  This TexasAF2579_2009_10_04 sequence meets the geographic criteria and genetic change prediction published on 2010-08-09.  The HA 230I in this case is combined with a second RBD polymorphism, 225E.

. . . . TexasAF2579_2009_10_04 (
. . . . . . . . syn20D,
. . . . . . . . 225E,
. . . . . . . . 230I (ATa),
. . . . . . . . syn246E [Texas77_E3_2009_10_06],
. . . . . . . . 264D,
. . . . . . . . 300S,
. . . . . . . . HA truncated after aa386)

Supporting Sequences

. . . . Texas77_E3_2009_10_06 (
. . . . . . . . 159S,
. . . . . . . . syn173G,
. . . . . . . . 219V,
. . . . . . . . 226R,
. . . . . . . . syn246E,
. . . . . . . . 275A,
. . . . . . . . 377K [H9N2])

. . . . TexasAF2589_2009_10_04 (
. . . . . . . . 159S,
. . . . . . . . syn173G,
. . . . . . . . 275A,
. . . . . . . . 377K [H9N2],
. . . . . . . . HA truncated after aa386)

. . . . TexasAF2627_2009_12_20 (
. . . . . . . . syn177L [H3N8, H6, H7N7],
. . . . . . . . . . . . . . [NagasakiHA_10_30_2010_03_29 with 513V, 550K,
. . . . . . . . . . . . . . NagasakiHA_10_27_2010_03_15 with 513V, 550K,
. . . . . . . . . . . . . . NY3230_2010_01_25 with syn213F,
. . . . . . . . . . . . . . Kansas25_2009_12_07 with 50A,
. . . . . . . . . . . . . . Indiana21_2009_12_01 with 212E, 313K,
. . . . . . . . . . . . . . SwedenGothenburg2_2009_12_01_xL with 146G,
. . . . . . . . . . . . . . Tambov_LLA_2009_11_30_xL_f with 225G,
. . . . . . . . . . . . . . Perm01_2009_11_25_xL_f,
. . . . . . . . . . . . . . MoscowOb_ZNF_2009_11_24_xL_f,
. . . . . . . . . . . . . . Ivanovo_KOE_2009_11_25_xL_f,
. . . . . . . . . . . . . . Astrakhan_CHRM_2009_11_24_xL_f,
. . . . . . . . . . . . . . GreeceThessaloniki2812_2009_11_22,
. . . . . . . . . . . . . . Ivanovo_STV1_2009_11_21 with 225G,
. . . . . . . . . . . . . . NorwayOslo110_2009_11_10,
. . . . . . . . . . . . . . MoscowOb_STV_2009_11_09_f with 225N,
. . . . . . . . . . . . . . GermanyNiedersachsen352_2009_11_05,
. . . . . . . . . . . . . . MoscowOb_OAM_2009_11_02_xL_f with 225G,
. . . . . . . . . . . . . . MoscowOb_DEI_2009_11_xL_f with 225G,
. . . . . . . . . . . . . . Ivanovo_AMV_2009_11_xL_f with 225G,
. . . . . . . . . . . . . . BelgiumBrussels106_2009_10_29,
. . . . . . . . . . . . . . Moscow_BVA_2009_10_24_xL_f with 225G,
. . . . . . . . . . . . . . SwedenUmea8_2009_10_07 with 0I, 244I,
. . . . . . . . . . . . . . Norway3364_2_2009_09_08_xL,
. . . . . . . . . . . . . . DenmarkAarhus82_2009_xL,
. . . . . . . . . . . . . . Orenburg_VNV_2009_08_06_xL,
. . . . . . . . . . . . . . England2800004_2009_07,
. . . . . . . . . . . . . . WiscD0008_2009_06_04],
. . . . . . . . 275A,
. . . . . . . . HA truncated after aa386)

Please visit GeneWurx.com for insight into the latest published studies.

Pune India Communicates Pandemic Genetic CrossTalk with England and United States

The practice of science exists to serve mankind by discovering answers. 

Utilising advanced tools, accurate observations and absolute logic, the mysterious becomes discernable and the discernable, in turn, becomes actionable.  Find a problem.  Fix a problem.

Tonight, we ask science to take a cue from a bouncing red ball, a small red ball.  Focus and faculty are requested.  We will paint a blue 50V on one small, red bouncing ball and then see where it goes.  If the results are plausible, we may try another.

Follow that springy, red ball from one continent to the next.  And then back again.

The ΣPF11 influenza reservoir is not mysterious in the least when the data that has been collected is actually made available for evaluation. Even post-pandemic publications may be informative.  We come to some such encounter today from a recent post-pandemic deposit of pandemic H1N1 sequences from England.

Watch the RnR * polymorphisms choose backgrounds and associate with other revisions as this Enhanced Acquisition virus becomes something new everyday in every city around the globe.  Humans, swine and birds are vectors, but only humans and birds can fly.  Swine store; humans and birds promote.

Let the following sequences describe the arc of two individual silent changes between and among India, the United Kingdom and the United States of America, including military personnel. Synonymous 50V and synonymous 348V are only two examples. These two representatives are certainly not comprehensive of this interchange behaviour.


. . . . England94840759_2009_11 (
. . . . . . . . syn50V [GDWujiang51_2010_03_18,
. . . . . . . . . . . . . . SouthCarolina02E3_2010_03_05
. . . . . . . . . . . . . . . . . . . . . . with 25R,
. . . . . . . . . . . . . . TexasJMS413_2010_02_03
. . . . . . . . . . . . . . . . . . . . . . with 25R,
. . . . . . . . . . . . . . TexasJMS391_2009_12_23
. . . . . . . . . . . . . . . . . . . . . . with 25R,
. . . . . . . . . . . . . . IndiaPune10604_2009_09],
. . . . . . . . 214R,
. . . . . . . . syn428L)

. . . . England734_2009_10 (
. . . . . . . . syn50V [GDWujiang51_2010_03_18,
. . . . . . . . . . . . . . SouthCarolina02E3_2010_03_05
. . . . . . . . . . . . . . . . . . . . . . with 25R,
. . . . . . . . . . . . . . TexasJMS413_2010_02_03
. . . . . . . . . . . . . . . . . . . . . . with 25R,
. . . . . . . . . . . . . . TexasJMS391_2009_12_23
. . . . . . . . . . . . . . . . . . . . . . with 25R,
. . . . . . . . . . . . . . IndiaPune10604_2009_09])

. . . . Eng662_2009_08 (
. . . . . . . . 25R [SouthCarolina02E3_2010_03_05
. . . . . . . . . . . . . . . .. . . . with syn50V,
. . . . . . . . . . . TexasJMS413_2010_02_03
. . . . . . . . . . . . . . . .. . . . with syn50V,
. . . . . . . . . . . TexasJMS391_2009_12_23
. . . . . . . . . . . . . . . .. . . . with syn50V,
. . . . . . . . . . . Texas extensive (10 more),
. . . . . . . . . . . Afghanistan (2),
. . . . . . . . . . . Russia149_2009_11_01_xL,
. . . . . . . . . . . Utah42_2009_07_24
. . . . . . . . . . . . . . . with 225G / 225N],
. . . . . . . . syn116R [NY6530_2010_02_21,
. . . . . . . . . . . . . . swOsaka1_2009_10,
. . . . . . . . . . . . . . Texas45071344_2009_09_07,
. . . . . . . . . . . . . . Portugal (2) 2009_08_19,
. . . . . . . . . . . . . . Military (30) 2009_08 to 2009_12,
. . . . . . . . . . . . . . Australia as early as 2009_07 (3),
. . . . . . . . . . . . . . SingTLL53_2009_06_14,
. . . . . . . . . . . . . . Columbia 2009_06 (2),
. . . . . . . . . . . . . . WiscD1606_2009_05_03],
. . . . . . . . syn348V [JapanAF2284_2009_11_27,
. . . . . . . . . . . . . . Frankfurt301_2009_11_23,
. . . . . . . . . . . . . . Ankara27_2009_11_11,
. . . . . . . . . . . . . . Yaroslavl_CHMV_2009_11_10_f with 225G,
. . . . . . . . . . . . . . Yakutsk_EAV_2009_11_18,
. . . . . . . . . . . . . . Thailand (24) as late as 2009_09_25,
. . . . . . . . . . . . . . BZ_SaoPaulo53823_2009_08_01_f,
. . . . . . . . . . . . . . Yakutsk_EAV_2009_11_18,
. . . . . . . . . . . . . . IndiaPune6196_2009_08,
. . . . . . . . . . . . . . IndiaDhule9433_2009_08,
. . . . . . . . . . . . . . IndiaPune807_2009_07,
. . . . . . . . . . . . . . PNG_PortMoresby2005_2009_07_08,
. . . . . . . . . . . . . . MoroccoRabat72_2009_07_02,
. . . . . . . . . . . . . . Slovenia2322_2009_07_01,
. . . . . . . . . . . . . . SingON442_2009_06_25,
. . . . . . . . . . . . . . ThailandCUB5_2009_06_13],
. . . . . . . . 494K [Indiana21_2009_12_10,
. . . . . . . . . . . . NewMexico07_2009_10_18,
. . . . . . . . . . . . NY5755_2009_10_01,
. . . . . . . . . . . . Ankara12_2009_09,
. . . . . . . . . . . . Ankara16_2009_09,
. . . . . . . . . . . . Ankara29_2009,
. . . . . . . . . . . . NY4568_2009_07_09,
. . . . . . . . . . . . NY4500_2009_07_01,
. . . . . . . . . . . . RhodeIsland04_2009_06_01])

Three American sequences are on file from December 2009 to March 2010 that accumulate both the 25R of Eng662_2009_08 and the syn50V of IndiaPune10604_2009_09. A pattern of attraction or permissiveness is suggested.

• SouthCarolina02E3_2010_03_05
• TexasJMS413_2010_02_03
• TexasJMS391_2009_12_23

Logic holds that we may expect similar transfer / cross-talk behaviour this year and that accumulation will occur again across the temporal axis. 

India is in the grip of a post-pandemic acceleration of death due to burgeoning caseloads and an apparent change in viral behaviour.  They have experience, foresight, materials and initiative, but the disease is overcoming the system and killing the citizens.  The health purveyors are doing everything by the book, but the chapters are out-dated apparently.

What will we find in those sequences?  How may we adapt our methods and materials to assist the families in India?  When will those viral changes be adopted in other countries?

Pondering is excellent, but action is better.

The sequences will tell the truth and provide a footing to act.  Please publish the sequences from May to August 2010.



Supporting / Related Sequences

. . . . India3725_2010_04_03 (
. . . . . . . . 117T,
. . . . . . . . syn167S,
. . . . . . . . 186P,
. . . . . . . . syn474C,
. . . . . . . . syn526S [SwedenMalmoe2_2010_05_07])

. . . . IndiaPune10604_2009_09 (
. . . . . . . . syn2N [UkrSumy797_2009_11_13_f,
. . . . . . . . . . . . . MongoliaJP5756_2009_10_15,
. . . . . . . .. . . . . SingON2416_2009_12_15],
. . . . . . . . syn50V [TexasJMS391_2009_12_23,
. . . . . . . . . . . . . . TexasJMS413_2009_02_03,
. . . . . . . . . . . . . . SouthCarolina02E3_2010_03_05],
. . . . . . . . syn178V (GTt) [NewHampshire02E3_2010_02_19 (GTa)],
. . . . . . . . 206S,
. . . . . . . . 296H,
. . . . . . . . syn348V [Yaroslavl_CHMV_2009_11_10_f with 225G,
. . . . . . . . . . . . . . Yakutsk_EAV_2009_11_18,
. . . . . . . . . . . . . . BZ_SaoPaulo53823_2009_08_01_f,
. . . . . . . . . . . . . . Eng662_2009_08],
. . . . . . . . syn485G [Nebraska 01_2010_01_28,
. . . . . . . . . . . . . . Orenburg2974_2009_11_16_xL,
. . . . . . . . . . . . . . NagasakiHA10_22_2010_03_01])

. . . . IndiaPune6196_2009_08 (
. . . . . . . . #12E,
. . . . . . . . syn2N [UkrSumy797_2009_11_13_f,
. . . . . . . . . . . . . MongoliaJP5756_2009_10_15,
. . . . . . . .. . . . . SingON2416_2009_12_15],
. . . . . . . . syn152I [NY6939_2009_12_11,
. . . . . . . . . . . . . . . tkOntarioFAV117_1C_2009_12_07,
. . . . . . . . . . . . . . . CalifVRDL115_2009_12_04,
. . . . . . . . . . . . . . . England1051_2009_12,
. . . . . . . . . . . . . . . England1050_2009_12,
. . . . . . . . . . . . . . . Scotland103_2009_12,
. . . . . . . . . . . . . . . England94800096_2009_11,
. . . . . . . . . . . . . . . ItalyAncona451_2009_11_27_f,
. . . . . . . . . . . . . . . NY6607_2009_11_24,
. . . . . . . . . . . . . . . CalifVRDL107_2009_11_15,
. . . . . . . . . . . . . . . CalifVRDL101_2009_11_05,
. . . . . . . . . . . . . . . DC_114_2009_11_04,
. . . . . . . . . . . . . . . Calif_SD35_2009_10_26,
. . . . . . . . . . . . . . . NY5447_2009_10_23,
. . . . . . . . . . . . . . . CalifVRDL84_2009_10_09,
. . . . . . . . . . . . . . . CalifVRDL87_2009_10_09,
. . . . . . . . . . . . . . . IndiaDhule9433_2009_08,
. . . . . . . . . . . . . . . SantoDomingoWR1057N_2009_07_02,
. . . . . . . . . . . . . . . SantoDomingoWR1058N_2009_07_02,
. . . . . . . . . . . . . . . SantoDomingoWR1059N_2009_06_30],
. . . . . . . . 206S,
. . . . . . . . 296H,
. . . . . . . . syn300P (CCt) [H5N1, H6N1, H7N3, H7N7],
. . . . . . . . . . . . . . . . . [Virginia24_2009_06_03 (CCt),
. . . . . . . . . . . . . . . . . NY0352_2009_12_27 (CCt),
. . . . . . . . . . . . . . . . . IllinoisAF2223_2009_12_18 (CCt),
. . . . . . . . . . . . . . . . . SingON2416_2009_12_15 (CCa),
. . . . . . . . . . . . . . . . . Vietnam2043_2009_12_01_TmX (CCa),
. . . . . . . . . . . . . . . . . HunanFur129_2010_01_13 (CCa),
. . . . . . . . . . . . . . . . . Slovenia5613_2009_12 (CCa),
. . . . . . . . . . . . . . . . . Minnesota40_2009_11_19 (CCa) w syn252V],
. . . . . . . . 342R,
. . . . . . . . syn348V [Yaroslavl_CHMV_2009_11_10_f with 225G,
. . . . . . . . . . . . . . Yakutsk_EAV_2009_11_18,
. . . . . . . . . . . . . . BZ_SaoPaulo53823_2009_08_01_f,
. . . . . . . . . . . . . . Eng662_2009_08],
. . . . . . . . syn355H [H2, H5N1, H6N1],
. . . . . . . . . . . . . . [GDLiwan1170_2010_03_14,
. . . . . . . . . . . . . . NY2963_2010_01_22
. . . . . . . . . . . . . .. . . . . . . . with 50E, 100N,
. . . . . . . . . . . . . . ItalyAncona508_2009_12_31
. . . . . . . . . . . . . .. . . . . . . . with syn181G,
. . . . . . . . . . . . . . Yamagata803_2009_12_21,
. . . . . . . . . . . . . . Astrakhan_CHRM_2009_11_24_xL_f,
. . . . . . . . . . . . . . MoscowOb_ZNF_2009_11_24_xL_f,
. . . . . . . . . . . . . . Ivanovo_STV1_2009_11_21
. . . . . . . . . . . . . .. . . . . . . . with 225G,
. . . . . . . . . . . . . . FloridaPensacola107_2009_11_12,
. . . . . . . . . . . . . . MoscowOb_STV_2009_11_09_f
. . . . . . . . . . . . . .. . . . . . . . with 225N,
. . . . . . . . . . . . . . swTaiwanTD4119B2_2009_11_04,
. . . . . . . . . . . . . . ArgentinaHNRG44_2009_06_30
. . . . . . . . . . . . . .. . . . . . . .  with 71G,
. . . . . . . . . . . . . . ArgentinaHNRG39_2009_06_23
. . . . . . . . . . . . . .. . . . . . . .  with 259T,

. . . . . . . . . . . . . . WiscD0589_2009_06_03
. . . . . . . . . . . . . .. . . . . . . .  with syn178V])

. . . . IndiaDhule9433_2009_08 (
. . . . . . . . #12E,
. . . . . . . . syn2N [UkrSumy797_2009_11_13_f,
. . . . . . . . . . . . . MongoliaJP5756_2009_10_15,
. . . . . . . . . . . . . SingON2416_2009_12_15,
. . . . . . . . . . . . . IndiaPune10604_2009_09, et al],
. . . . . . . . syn152I [NY6939_2009_12_11,
. . . . . . . . . . . . . . . tkOntarioFAV117_1C_2009_12_07,
. . . . . . . . . . . . . . . CalifVRDL115_2009_12_04,
. . . . . . . . . . . . . . . England1051_2009_12,
. . . . . . . . . . . . . . . England1050_2009_12,
. . . . . . . . . . . . . . . Scotland103_2009_12,
. . . . . . . . . . . . . . . England94800096_2009_11,
. . . . . . . . . . . . . . . ItalyAncona451_2009_11_27_f,
. . . . . . . . . . . . . . . NY6607_2009_11_24,
. . . . . . . . . . . . . . . CalifVRDL107_2009_11_15,
. . . . . . . . . . . . . . . CalifVRDL101_2009_11_05,
. . . . . . . . . . . . . . . DC_114_2009_11_04,
. . . . . . . . . . . . . . . Calif_SD35_2009_10_26,
. . . . . . . . . . . . . . . NY5447_2009_10_23,
. . . . . . . . . . . . . . . CalifVRDL84_2009_10_09,
. . . . . . . . . . . . . . . CalifVRDL87_2009_10_09,
. . . . . . . . . . . . . . . IndiaPune6196_2009_08,
. . . . . . . . . . . . . . . SantoDomingoWR1057N_2009_07_02,
. . . . . . . . . . . . . . . SantoDomingoWR1058N_2009_07_02,
. . . . . . . . . . . . . . . SantoDomingoWR1059N_2009_06_30],
. . . . . . . . 176I,
. . . . . . . . 205W,
. . . . . . . . 206S,
. . . . . . . . 252L (cTG) [Maryland03_2010_01_13 (tTG),
. . . . . . . . . . . . . . . Guangdong2361_2009_11_25 (tTG)],
. . . . . . . . 296H,
. . . . . . . . syn348V [Yaroslavl_CHMV_2009_11_10_f with 225G,
. . . . . . . . . . . . . . Yakutsk_EAV_2009_11_18,
. . . . . . . . . . . . . . BZ_SaoPaulo53823_2009_08_01_f,
. . . . . . . . . . . . . . Eng662_2009_08],
. . . . . . . . syn386I [1918, Unique to PF11])

. . . . IndiaPune807_2009_07 (
. . . . . . . . #12E,
. . . . . . . . syn2N [UkrSumy797_2009_11_13_f ,
. . . . . . . . . . . . . MongoliaJP5756_2009_10_15,
. . . . . . . .. . . . . SingON2416_2009_12_15],
. . . . . . . . 100Q [Unique to PF11],
. . . . . . . . syn104L [CalifVRDL132_2009_12_30,
. . . . . . . . . . . . . . . Texas45122538_2009_09_12,
. . . . . . . . . . . . . . . WiscD0410_2009_09_24,
. . . . . . . . . . . . . . . WiscD1355_2009_09_21],
. . . . . . . . 206S,
. . . . . . . . 296H,
. . . . . . . . syn348V [Yaroslavl_CHMV_2009_11_10_f with 225G,
. . . . . . . . . . . . . . Yakutsk_EAV_2009_11_18,
. . . . . . . . . . . . . . BZ_SaoPaulo53823_2009_08_01_f,
. . . . . . . . . . . . . . Eng662_2009_08],
. . . . . . . . 461E [Unique to PF11]),

. . . . England1048_2009_12 (
. . . . . . . . 214R,
. . . . . . . . 225E,
. . . . . . . . 324I)

. . . . England1051_2009_12 (
. . . . . . . . syn10Y,
. . . . . . . . 35I,
. . . . . . . . syn152I [NY6939_2009_12_11,
. . . . . . . . . . . . . . . tkOntarioFAV117_1C_2009_12_07,
. . . . . . . . . . . . . . . CalifVRDL115_2009_12_04,
. . . . . . . . . . . . . . . England1050_2009_12,
. . . . . . . . . . . . . . . Scotland103_2009_12,
. . . . . . . . . . . . . . . England94800096_2009_11,
. . . . . . . . . . . . . . . ItalyAncona451_2009_11_27_f,
. . . . . . . . . . . . . . . NY6607_2009_11_24,
. . . . . . . . . . . . . . . CalifVRDL107_2009_11_15,
. . . . . . . . . . . . . . . CalifVRDL101_2009_11_05,
. . . . . . . . . . . . . . . DC_114_2009_11_04,
. . . . . . . . . . . . . . . Calif_SD35_2009_10_26,
. . . . . . . . . . . . . . . NY5447_2009_10_23,
. . . . . . . . . . . . . . . CalifVRDL84_2009_10_09,
. . . . . . . . . . . . . . . CalifVRDL87_2009_10_09,
. . . . . . . . . . . . . . . IndiaPune6196_2009_08,
. . . . . . . . . . . . . . . IndiaDhule9433_2009_08,
. . . . . . . . . . . . . . . SantoDomingoWR1057N_2009_07_02,
. . . . . . . . . . . . . . . SantoDomingoWR1058N_2009_07_02,
. . . . . . . . . . . . . . . SantoDomingoWR1059N_2009_06_30],
. . . . . . . . 206S,
. . . . . . . . syn219I,
. . . . . . . . syn254P,
. . . . . . . . syn276H,
. . . . . . . . syn456L,
. . . . . . . . 463V,
. . . . . . . . 502K [Ukraine],
. . . . . . . . 523A)

. . . . SouthCarolina02E3_2010_03_05 (
. . . . . . . . 25R [Utah42_2009_07_24 with 225G / 225N,
. . . . . . . . . . . Texas extensive (12),
. . . . . . . . . . . Afghanistan (2),
. . . . . . . . . . . Russia149_2009_11_01_xL],
. . . . . . . . syn50V [TexasJMS391_2009_12_23,
. . . . . . . . . . . . . TexasJMS413_2009_02_03,
. . . . . . . . . . . . . IndiaPune10604_2009_09],
. . . . . . . . 130E (GAa) [1918],
. . . . . . . . . . . . [Netherlands2243_2009_11_17_xL (GAg) mix
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . with 158E, 225G,
. . . . . . . . . . . . Iraq8531_2009_09_08_xL (GAa) with 131L,
. . . . . . . . . . . . Guangdong0872_2009_12_27 (GAa),
. . . . . . . . . . . . SingTLL52_2009_06_14 (GAa),
. . . . . . . . . . . . swAlbertaOTH_33_23_2009_05_03 (GAa)],
. . . . . . . . 206T)

. . . . TexasJMS413_2010_02_03 (
. . . . . . . . 25R,
. . . . . . . . syn50V,
. . . . . . . . syn84E)

. . . . TexasJMS391_2009_12_23 (
. . . . . . . . 25R,
. . . . . . . . syn50V,
. . . . . . . . syn84E,
. . . . . . . . syn374A)

. . . . NY2963_2010_01_22 (
. . . . . . . . 50E,
. . . . . . . . 100N,
. . . . . . . . syn270I,
. . . . . . . . syn291T,
. . . . . . . . syn355H,
. . . . . . . . 377K)

. . . . FloridaPensacola107_2009_11_12 (
. . . . . . . . 35I,
. . . . . . . . syn338G,
. . . . . . . . syn355H,
. . . . . . . . syn456L)

. . . . WiscD0589_2009_06_03 (
. . . . . . . . syn36L,
. . . . . . . . syn178V,
. . . . . . . . 193N,
. . . . . . . . syn355H)

. . . . Indiana21_2009_12_01 (
. . . . . . . . syn12A,
. . . . . . . . syn177L,
. . . . . . . . syn193S,
. . . . . . . . 212E mix,
. . . . . . . . 313K,
. . . . . . . . syn346G,
. . . . . . . . 359G,
. . . . . . . . 377K,
. . . . . . . . 494K)

. . . . IllinoisAF2223_2009_12_18 (
. . . . . . . . syn34N,
. . . . . . . . syn300P (CCt),
. . . . . . . . 377K,
. . . . . . . . HA truncated after aa386)

. . . . CalifVRDL107_2009_11_15 (
. . . . . . . . 35I,
. . . . . . . . 39N,
. . . . . . . . syn152I,
. . . . . . . . 206S,
. . . . . . . . syn219I,
. . . . . . . . syn276H,
. . . . . . . . syn456L,
. . . . . . . . 463V,
. . . . . . . . 523A)

. . . . CalifVRDL115_2009_12_04 (
. . . . . . . . 35I,
. . . . . . . . 77N,
. . . . . . . . syn152I,
. . . . . . . . 206S,
. . . . . . . . syn219I,
. . . . . . . . syn276H,
. . . . . . . . syn456L,
. . . . . . . . 463V,
. . . . . . . . 523A)

. . . . Malmoe2_2010_05_07 (
. . . . . . . . 89G,
. . . . . . . . syn108L [tn],
. . . . . . . . syn159N,
. . . . . . . . 206T,
. . . . . . . . 259T [ArgentinaHNRG39_2009_06_23
. . . . . . . . . . . . . . . . . . . .  with syn355H],
. . . . . . . . syn280T [1918, WSN33],
. . . . . . . . syn526S [India3725_2010_04_03])

. . . . ItalyAncona508_2009_12_31 (
. . . . . . . . syn172K,
. . . . . . . . syn181G,
. . . . . . . . syn239P,
. . . . . . . . syn355H,
. . . . . . . . 373H)

. . . . GDLiwan1170_2010_03_14 (
. . . . . . . . 131P,
. . . . . . . . 202A,
. . . . . . . . 271T,
. . . . . . . . 298V,
. . . . . . . . syn355H,
. . . . . . . . 375F)

. . . . tkOntarioFAV117_1C_2009_12_07 (
. . . . . . . . 35I [H5N1],
. . . . . . . . syn55L,
. . . . . . . . syn152I,
. . . . . . . . 186P,
. . . . . . . . syn205G,
. . . . . . . . syn219I,
. . . . . . . . syn276H [1918, S5],
. . . . . . . . syn456L,
. . . . . . . . 463V,
. . . . . . . . syn486T,
. . . . . . . . 523A)

Please refer to additional studies for further genetic analyses, including the survey on amino acid revisions potentially related to Vaccine Escape.  A hyper-morphic, TamiFlu-resistant sequence from the state of Washington in Spring 2010 has recently been profiled with similar zoonotic influences.


This analysis was fueled by puneri amti and funded with contributions from concerned families, including HOD, BMS, MJV and CSB.


* Rare, not Random

Please visit GeneWurx.com for insight into the latest published studies.

4,069 People Began TamiFlu Treatment on Monday in Pune India

If someone had told the citizens that the virus would be post-pandemic on Thursday, they may have asked for a treatment deferral . . . unless they trusted their own eyes.  No press release can revise the rise of a IDEARRV disease like the ΣPF11 influenza reservoir.

The facts speak for themselves.

On Monday, 2010-08-09, more than 4,000 citizens were placed on TamiFlu treatment in the city of Pune, India.  83 deaths were recorded from pre-post-pandemic influenza for the prior 7 days in India. 

For the country of India, the weekly average confirmed Influenza death count over the past 14 months is slightly above 30 deaths per week.  The fatality count from the past week of 83 is 2.7 times the average rate, a substantial acceleration.  The Case Fatality Rate varies from 8.8% to 12.1% in the city/district based on the source of data and date bounding. Roughly 1 in 10 people with a confirmed influenza test die in India.

More than 75% of the deaths are in the 20-49 year old age bracket according to the raw data we have been able to reference, with reports from others as low as 70%.  Years of Life Lost calculations defy all "normal" influenza trends.  Traditional influenza has a CFR of less than half a percent and more than 98% of that half percent are over 65 or under 2 years old. These numbers from India in 2010 are not suggestive of a post-pandemic status.

Perhaps we should all just re-base now that we have read the press releases and settle ourselves into this post-pandemic era.

Step 1. We could  call 83 deaths per week the new "normal" and post that idea as the rate for "seasonal" influenza.  Our forms and percentages will then be quite less concerning to the public.  Then we could take a note from the public health textbooks and "estimate using the most advanced models" a normal CFR and game the model variables until we evaluate to the current 8.8% CFR of India. 

Step 2. Combine the power of the press with the power of the "expert" credential, even perhaps a committee of experts?  In a few pen strokes and an hour here and there behind a microphone, the public would be assuaged and all could return to "business as usual".

Or we could hear the public whose health is suffering, read the data and then tell the truth.

• 4,000 on TamiFlu treatment in one day in one city 
• 83 confirmed deaths in one week across India 
• Post-Pandemic

One of these things is not like the other?

Though we'd like to see the sequences from these fatal cases that are currently accelerating throughout India, a review of the data on file will allow insight into the building / budding process of a pandemic virus into and out of a monsoon climate. An array of FlightPaths may be detected that led to Hydra strains downstream in time. We are under no uncertainty that the viral reservoir will follow a similar route this year.

India, last year, was a testing field for PF11. In a nutshell, the sub-continent had a depth of uptake from Asia and North America that transformed and promoted to Europe and then returned to the United States. That pathway is a generalisation / characterisation and is not comprehensive.  Suspicions do lead to a repeat of last year.

Has, perhaps, the 225G on the HA and the 275Y on the NA become more substantial in India after travelling through the world? Is HA 225N playing a role in the more rapid expirations, while cross-linked 225G causes the lingering 3-5 day, ICU-bound feats of ultimate exhaustion? Is a deep, immune escape Hydra carrying HA 165N and 230I at work in the districts showing a 20-29% Case Fatality Rate?

We watch as vaccinated doctors are admitted to critical care wards, as hospitals with advanced experience and materials lose patients daily to Influenza, even upon using immediate TamiFlu blanketing.  Reports of fatalities associated with caregivers, including doctors, are demonstrated in multiple geographies.

Review the sequences that have been made available. See the truth.
 
. . . . India3725_2010_04_03 (
. . . . . . . . 117T,
. . . . . . . . syn167S,
. . . . . . . . 186P,
. . . . . . . . syn474C,
. . . . . . . . syn526S [Malmoe2_2010_05_07 with 89G])

. . . . IndiaPune10278_2009_09 (
. . . . . . . . syn48R [Norway1359_2009_05_16,
. . . . . . . . . . . . . . UkrZakarpatska830_2009_11_12_f, et al],
. . . . . . . . syn131S [Russia, Afghan, Kenya,
. . . . . . . . . . . . . . NY6943_2009_12_07_xL,
. . . . . . . . . . . . . . Wisconsin, California,
. . . . . . . . . . . . . . Nicaragua, Greece],
. . . . . . . . 206T,
. . . . . . . . 225G,
. . . . . . . . syn413K [H9N2],
. . . . . . . . 454N [H7N3, H7N7, H9N2]
. . . . . . . . . . . . . . [IndiaPune9355_2009_08 with 225G,
. . . . . . . . . . . . . . IndiaBlore236_2009_06_xL with 226R,
. . . . . . . . . . . . . . SouthCarolina18_2009_09_16_VxX,
. . . . . . . . . . . . . . FL_Pen210_2009_11_10],
. . . . . . . . 550T [Unique to India])

. . . . IndiaPune10604_2009_09 (
. . . . . . . . syn2N [UkrSumy797_2009_11_13_f,
. . . . . . . . . . . . . MongoliaJP5756_2009_10_15,
. . . . . . . .. . . . . SingON2416_2009_12_15],
. . . . . . . . syn50V [TexasJMS391_2009_12_23,
. . . . . . . . . . . . . . TexasJMS413_2009_02_03,
. . . . . . . . . . . . . . SouthCarolina02E3_2010_03_05],
. . . . . . . . syn178V (GTt) [NewHampshire02E3_2010_02_19 (GTa)],
. . . . . . . . 206S,
. . . . . . . . 296H,
. . . . . . . . syn348V [Yaroslavl_CHMV_2009_11_10_f with 225G,
. . . . . . . . . . . . . . Yakutsk_EAV_2009_11_18,
. . . . . . . . . . . . . . BZ_SaoPaulo53823_2009_08_01_f,
. . . . . . . . . . . . . . Eng662_2009_08],
. . . . . . . . syn485G [Nebraska 01_2010_01_28,
. . . . . . . . . . . . . . Orenburg2974_2009_11_16_xL,
. . . . . . . . . . . . . . NagasakiHA10_22_2010_03_01])

. . . . IndiaDelhi3704_2009_09 (
. . . . . . . . syn44L,
. . . . . . . . syn106E,
. . . . . . . . syn125S,
. . . . . . . . syn159N,
. . . . . . . . 206T,
. . . . . . . . syn297N,
. . . . . . . . syn413K)

. . . . IndiaNsk10348_2009_09 (
. . . . . . . .  #12E,
. . . . . . . . syn33V,
. . . . . . . . 206S,
. . . . . . . . 225G,
. . . . . . . . 296H,
. . . . . . . . syn406R,
. . . . . . . . syn439D)

. . . . IndiaJalna9436_2009_08_30  (
. . . . . . . . 8V [Yaroslavl_KMP_2009_08_25,
. . . . . . . . . .  NY7216_2009_12_21 with 225G,
. . . . . . . . . .  Utah32_2009_10_14],
. . . . . . . . syn147F [H3N8 Avian, H4, H5, H6, H11],
. . . . . . . . . . . . . . [Yaroslavl_KMP_2009_08_25,
. . . . . . . . . . . . . . IndiaPune21115_2009_12,
. . . . . . . . . . . . . . Athens943_2009_06_12,
. . . . . . . . . . . . . . CalifVRDL99_2009_11_05,
. . . . . . . . . . . . . . Texas44301765_2009_08_30],
. . . . . . . . syn210S,
. . . . . . . . 226R)

. . . . IndiaDelhi3610_2009_08 (
. . . . . . . . syn125S,
. . . . . . . . 157E,
. . . . . . . . 206T,
. . . . . . . . syn294P,
. . . . . . . . syn464G)

. . . . IndiaMum9312_2009_08 (
. . . . . . . . 208K,
. . . . . . . . 225G,
. . . . . . . . syn385V)

. . . . IndiaPune6196_2009_08 (
. . . . . . . . #12E,
. . . . . . . . syn2N [UkrSumy797_2009_11_13_f,
. . . . . . . . . . . . . MongoliaJP5756_2009_10_15,
. . . . . . . .. . . . . SingON2416_2009_12_15],
. . . . . . . . syn152I,
. . . . . . . . 206S,
. . . . . . . . 296H,
. . . . . . . . syn300P (CCt) [H5N1, H6N1, H7N3, H7N7],
. . . . . . . . . . . . . . . . . [Virginia24_2009_06_03 (CCt),
. . . . . . . . . . . . . . . . . NY0352_2009_12_27 (CCt),
. . . . . . . . . . . . . . . . . SingON2416_2009_12_15 (CCa),
. . . . . . . . . . . . . . . . . Vietnam2043_2009_12_01_TmX (CCa),
. . . . . . . . . . . . . . . . . HunanFur129_2010_01_13 (CCa),
. . . . . . . . . . . . . . . . . Slovenia5613_2009_12 (CCa),
. . . . . . . . . . . . . . . . . Minnesota40_2009_11_19 (CCa) w syn252V],
. . . . . . . . 342R,
. . . . . . . . syn348V [Yaroslavl_CHMV_2009_11_10_f with 225G,
. . . . . . . . . . . . . . Yakutsk_EAV_2009_11_18,
. . . . . . . . . . . . . . BZ_SaoPaulo53823_2009_08_01_f,
. . . . . . . . . . . . . . Eng662_2009_08],
. . . . . . . . syn355H [H2, H5N1, H6N1],
. . . . . . . . . . . . . . [MoscowOb_ZNF_2009_11_24_xL_f,
. . . . . . . . . . . . . . MoscowOb_STV_2009_11_09_f with 225N,
. . . . . . . . . . . . . . Ivanovo_STV1_2009_11_21 with 225G,
. . . . . . . . . . . . . . WiscD0589_2009_06_03])

. . . . IndiaPune9355_2009_08 (
. . . . . . . . #10T,
. . . . . . . . syn#4Y [GermanyBY74_2009 with 225G & 226R,
. . . . . . . . . . . . . FL_Pensacola40_2009_11_09],
. . . . . . . . 206T,
. . . . . . . . 225G,
. . . . . . . . syn372Q [H2, H3N8, H4, H5, H6, H7N3, H7N7, H9N2, H11]
. . . . . . . . . . . . . . [IndiaBlore236_2009_06_xL with 226R,
. . . . . . . . . . . . . . Ulyanovsk_SHTA_2009_10_31_f_225N,
. . . . . . . . . . . . . . Orenburg2974_2009_11_16_xL,
. . . . . . . . . . . . . . Russia fatal extensive,
. . . . . . . . . . . . . . swIowa35572_2009_12_16,
. . . . . . . . . . . . . . NagasakiHA10_22_2010_03_01],
. . . . . . . . 373K,
. . . . . . . . syn413K [H9N2],
. . . . . . . . 454N [H7N3, H7N7, H9N2]
. . . . . . . . . . . . .  [IndiaBlore236_2009_06 with 226R,
. . . . . . . . . . . . .  SouthCarolina18_2009_09_16_VxX,
. . . . . . . . . . . . .  FL_Pen210_2009_11_10],
. . . . . . . . 550T [Unique to India])

. . . . IndiaDhule9433_2009_08 (
. . . . . . . . #12E,
. . . . . . . . syn2N [UkrSumy797_2009_11_13_f,
. . . . . . . . . . . . . MongoliaJP5756_2009_10_15,
. . . . . . . . . . . . . SingON2416_2009_12_15,
. . . . . . . . . . . . . IndiaPune10604_2009_09, et al],
. . . . . . . . syn152I,
. . . . . . . . 176I,
. . . . . . . . 205W,
. . . . . . . . 206S,
. . . . . . . . 252L (cTG) [Maryland03_2010_01_13 (tTG),
. . . . . . . . . . . . . . . Guangdong2361_2009_11_25 (tTG)],
. . . . . . . . 296H,
. . . . . . . . syn348V [Yaroslavl_CHMV_2009_11_10_f with 225G,
. . . . . . . . . . . . . . Yakutsk_EAV_2009_11_18,
. . . . . . . . . . . . . . BZ_SaoPaulo53823_2009_08_01_f,
. . . . . . . . . . . . . . Eng662_2009_08],
. . . . . . . . syn386I [1918, Unique to PF11])

. . . . IndiaBlore310_2009_07 (
. . . . . . . . syn105R,
. . . . . . . . syn112S,
. . . . . . . . 206T,
. . . . . . . . syn477T)

. . . . IndiaPune807_2009_07 (
. . . . . . . . #12E,
. . . . . . . . syn2N [UkrSumy797_2009_11_13_f ,
. . . . . . . . . . . . . MongoliaJP5756_2009_10_15,
. . . . . . . .. . . . . SingON2416_2009_12_15],
. . . . . . . . 100Q [Unique to PF11],
. . . . . . . . syn104L [CalifVRDL132_2009_12_30,
. . . . . . . . . . . . . . . Texas45122538_2009_09_12,
. . . . . . . . . . . . . . . WiscD0410_2009_09_24,
. . . . . . . . . . . . . . . WiscD1355_2009_09_21],
. . . . . . . . 206S,
. . . . . . . . 296H,
. . . . . . . . syn348V [Yaroslavl_CHMV_2009_11_10_f with 225G,
. . . . . . . . . . . . . . Yakutsk_EAV_2009_11_18,
. . . . . . . . . . . . . . BZ_SaoPaulo53823_2009_08_01_f,
. . . . . . . . . . . . . . Eng662_2009_08],
. . . . . . . . 461E [Unique to PF11])

. . . . IndiaBlore236_2009_06_xL (
. . . . . . . . 226R,
. . . . . . . . syn372Q [H2, H3N8, H4, H5, H6, H7N3, H7N7, H9N2, H11]
. . . . . . . . . . . . . . [IndiaPune9355_2009_08 with 225G,
. . . . . . . . . . . . . . Ulyanovsk_SHTA_2009_10_31_f_225N,
. . . . . . . . . . . . . . Orenburg2974_2009_11_16_xL,
. . . . . . . . . . . . . . Russia fatal extensive,
. . . . . . . . . . . . . . swIowa35572_2009_12_16,
. . . . . . . . . . . . . . NagasakiHA10_22_2010_03_01]),
. . . . . . . . syn413K [H9N2],
. . . . . . . . 454N [H7N3, H7N7, H9N2]
. . . . . . . . . . . . . . [IndiaPune9355_2009_08 with 225G,
. . . . . . . . . . . . . . SouthCarolina18_2009_09_16_VxX,
. . . . . . . . . . . . . . FL_Pen210_2009_11_10],
. . . . . . . . 550T [Unique to India])

. . . . IndiaHyderabad51_2009_05 (
. . . . . . . . 1T,
. . . . . . . . syn74S [swNC13598_2010_03_09_xL_f
. . . . . . . . . . . . . . . . . . with 15 HA + 4 NA revisions],
. . . . . . . . syn144A (GCc) [Rome632_2009_11_23 (GCg)],
. . . . . . . . 206S)

. . . . equineIndiaAhmedabad1_2009_04_26 (H3N8) (
. . . . . . . . syn211K [H3N8],
. . . . . . . . . . . . . . [MXinDRE50617_2009_11_01 with 225G,
. . . . . . . . . . . . . . Niedersachsen330_2009_07_20],
. . . . . . . . et al)

. . . . equineIndiaKatraJammu7_2008_06 (H3N8) (
. . . . . . . . syn211K [H3N8],
. . . . . . . . . . . . . . [MXinDRE50617_2009_11_01 with 225G,
. . . . . . . . . . . . . . Niedersachsen330_2009_07_20],
. . . . . . . . et al)

Please refer to additional studies for further genetic analyses, including the survey on amino acid revisions potentially related to Vaccine Escape.  A hyper-morphic, TamiFlu-resistant sequence from the state of Washington in Spring 2010 has recently been profiled with similar zoonotic influences.


This analysis was fueled by baingan bharta and funded with contributions from concerned families, including HOD, BMS, MJV and CSB.

Please visit GeneWurx.com for insight into the latest published studies.

Ukraine 502K Marker from Cross-Linked Fatality Documented Worldwide, including England, the United States, Australia and Singapore

The UK Centre for Infections, Health Protection Agency provided a very large deposit marked 2010-08-12 this week. Among numerous RnR * examples describing flow of particular genetic markers, one change from the Ukraine high fatality circumstance of late 2009 becomes documented in England.

The HA 502K found on the UkrTernopilN10_2009_10_28_xL_f cross-linked fatality insinuated into England in only days after this sentinel Ukraine infection was sampled and isolated. A downstream HA revision, 504N, is also documented in this same deposit as appearing widely in England with one instance in Scotland.

. . . . England94880537_2009_11 (
. . . . . . . . 35I,
. . . . . . . . 206T,
. . . . . . . . syn244T,
. . . . . . . . syn490P,
. . . . . . . . 502K)

. . . . England1051_2009_12 (
. . . . . . . . syn10Y [China22811_2009_12
. . . . . . . . . . . . . . . . . . . . . with 14 HA revisions],
. . . . . . . . 35I,
. . . . . . . . syn152I,
. . . . . . . . 206S,
. . . . . . . . syn219I,
. . . . . . . . syn254P,
. . . . . . . . syn276H,
. . . . . . . . syn456L,
. . . . . . . . 463V,
. . . . . . . . 502K,
. . . . . . . . 523A)

. . . . . . . . 502K
. . . . . . . . . . . [Oz_Victoria800_2010_06_02,
. . . . . . . . . . . SingGP2362_2010_05_18,
. . . . . . . . . . . CalifVRDL2_2010_01_11,
. . . . . . . . . . . GuangxiLongan1870_2009_12_16,
. . . . . . . . . . . AlgeriaG2902_2009_12_09 mix with 225E,
. . . . . . . . . . . Egypt114_2009_12_06 with 225E,
. . . . . . . . . . . England1051_2009_12,
. . . . . . . . . . . England94880537_2009_11,
. . . . . . . . . . . Scandinavia (7),
. . . . . . . . . . . UkrTernopilN10_2009_10_28_xL_f with 225G,
. . . . . . . . . . . Haiti534_2009_10_19,
. . . . . . . . . . . SouthCarolina38_2009_09_17,
. . . . . . . . . . . SouthCarolina36_2009_09_02,
. . . . . . . . . . . Jiangyin34_2009_08_16,
. . . . . . . . . . . Michigan21_2009_08_07,
. . . . . . . . . . . California33_2009_08_06,
. . . . . . . . . . . ThailandTHB0438_2009_07_28,
. . . . . . . . . . . JapanPR1070_2009_07_10])

Please refer to additional studies for further genetic analyses, including the survey on amino acid revisions potentially related to Vaccine Escape.  A hyper-morphic, TamiFlu-resistant sequence from the state of Washington in Spring 2010 has recently been profiled with similar zoonotic influences.

* Rare, not Random

Please visit GeneWurx.com for insight into the latest published studies.

Mid-Life Travels of HA 275A, A Companion of the 230I Receptor Binding Domain Revision

Last Updated 2010-11-16

Apparently, friends travel together.

But not always in exactly the same passenger car, yet frequently on the same train.

Long trips may be passed in tolerable companionship that may involve discretion on one day and deepening fondness on the next, depending on the prevailing circumstances of the moment. 

Imagine a day on the railway . . . four friends, one accompanied with a new acquaintance.  In the afternoon, the initiate may rest while the others get some fresh air on the back of the dining car. After 10 minutes of glorious landscape, a howling wind breaks the party into timid and bold, with the bold holding ground until soaked by an instant shower. That mutual embarrassment may align the two rinsed travellers more than once on the duration of this trip for, after all, folly loves a jest. 

Sometimes, the oddest of circumstances, the unexpected, harnesses an attraction.

At 4 P.M., the two sensible travellers may take tea together while the others repose themselves to separate rooms for mid-day study. The entire comaraderie, finding themselves famished at the dinner hour and in want of company, may unsettle the maitre'd.  Practically re-orienting the room, pushing tables together, bustling in and out of the other passengers without such companionship, they eat and drink while recalling one to the other all the fine destinations they've visited.  With a toast, they tell tales and communicate their paths of felicity to the others.  Communication spawns ideas and enlivens standing plans.

Apparently, many of the RnR * polymorphisms that are found in this pandemic are friends . . . polite acquaintances at the bare minimum.  In communication.  Underway.

A virus does not halt when mere mankind votes and resolves that a pandemic is complete.  The virus that spawned the 1918 tragic waves, killing 50 to 100 million people, continued in progress for at least 13 years in humans and is yet at work today.  Have you read the press releases from that era of unapologised public health failure? 

Resolve yourself that pandemic viral reservoirs do not read press releases, nor relent at the political stoke of a pen.

Let the observational science do the talking. 

Read the sequences.

. . . . GuamNHRC0016_2009_09_04 (
. . . . . . . . 182V [1918, tn, H3N8, H4, H6N1, H7N3, H11],
. . . . . . . . . . . . . [NY7480_2010_03_01
. . . . . . . . . . . . . . . . . with 5 Avian Influenza polymorphisms,
. . . . . . . . . . . . . Boston103_2009_06_15],
. . . . . . . . syn231N [NY3230_2010_01_25
. . . . . . . . . . . . . . . . . with 100N, 159K, syn213F, syn177L,
. . . . . . . . . . . . . . Lisboa104_2009_12_22,
. . . . . . . . . . . . . . SingaporeGP4309_2009_11_12 with 377K,
. . . . . . . . . . . . . . HondurasAF2214_2009_10_20,
. . . . . . . . . . . . . . CalifVRDL36_2009_09_24 with 377K,
. . . . . . . . . . . . . . JapanNHRC0004_2009_08_04,
. . . . . . . . . . . . . . GuamNHRC0010_2009_07_21, et al],
. . . . . . . . 275A mix,
. . . . . . . . . . . . . [First Found in
. . . . . . . . . . . . . Stockholm35_2009_06_01
. . . . . . . . . . . . . traveler from United States],
. . . . . . . . syn538F [NY7426_2009_12_08 with 0I,
. . . . . . . . . . . . . . JapanNHRC0004_2009_08_04,
. . . . . . . . . . . . . . GuamNHRC0010_2009_07_21, et al]),

. . . . CalifAF2123_2009_10_14 (
. . . . . . . . 275A [First Found in
. . . . . . . . . . . . Stockholm35_2009_06_01
. . . . . . . . . . . . traveler from United States],
. . . . . . . . 377K,
. . . . . . . . HA truncated after aa386)

. . . . ColoradoAF2140_2009_10_06 (
. . . . . . . . syn66G [China (2) 2010_02_02 & 02_23,
. . . . . . . . . . . . . TexasJMS404_2010_01_08 with syn179L,
. . . . . . . . . . . . . TexasJMS401_2010_01_03 with 377K,
. . . . . . . . . . . . . Hawaii (2) 2010_01_02 & 01_03,
. . . . . . . . . . . . . Cyprus5494_2009_12_16,
. . . . . . . . . . . . . Arizona20_CXC1_2009_12_06,
. . . . . . . . . . . . . Nepal (2) beginning 2009_11_16,
. . . . . . . . . . . . . TverIIV2969_2009_11_14_xl_f with 225G,
. . . . . . . . . . . . . Japan (3) beginning 2009_10_23,
. . . . . . . . . . . . . England93280011_2009_08,
. . . . . . . . . . . . . Ancona17_2009_07_17,
. . . . . . . . . . . . . WiscS0208_2009_06_09],
. . . . . . . . 166N,
. . . . . . . . 275A [First Found in
. . . . . . . . . . . . Stockholm35_2009_06_01
. . . . . . . . . . . . traveler from United States],
. . . . . . . . HA truncated after aa386)

Changes at and around amino acid 275 are associated with M230I conversion in the Receptor Binding Domain.  275A is strongly associated.

Two sequences from the US, NY3230_2010_01_25 and Indiana21_2009_12_01, provide potential for interchange between the Russian / Eastern European sequences carrying syn177L and the recently profiled emerging sub-clade from the US and the Ukraine during April to June 2010.

Supporting Sequences

. . . . Finland655_2009_10_20 (
. . . . . . . . syn120F,
. . . . . . . . 275A,
. . . . . . . . 317M [Finland,
. . . . . . . . . . . . . Athens,
. . . . . . . . . . . . . Kuwait,
. . . . . . . . . . . . . Japan,
. . . . . . . . . . . . . Kansas,
. . . . . . . . . . . . . Texas,
. . . . . . . . . . . . . Wisconsin])

. . . . Finland665_2009_10_27 (
. . . . . . . . syn120F,
. . . . . . . . 275A,
. . . . . . . . 317M [Finland,
. . . . . . . . . . . . . Athens,
. . . . . . . . . . . . . Kuwait,
. . . . . . . . . . . . . Japan,
. . . . . . . . . . . . . Kansas,
. . . . . . . . . . . . . Texas,
. . . . . . . . . . . . . Wisconsin])

. . . . Finland2_2010_01_12 (
. . . . . . . . syn177L,
. . . . . . . . 222R,
. . . . . . . . 233H,
. . . . . . . . syn296Q,
. . . . . . . . 324I,
. . . . . . . . syn413K)

. . . . GreeceLivadia1851_2010_01_26 (
. . . . . . . . 132D,
. . . . . . . . syn177L,
. . . . . . . . 196L,
. . . . . . . . syn221P,
. . . . . . . . 222Q,
. . . . . . . . 276Q,
. . . . . . . . 373H,
. . . . . . . . syn420D)

Please refer to additional studies for further genetic analyses, including the survey on amino acid revisions potentially related to Vaccine Escape.  A hyper-morphic, TamiFlu-resistant sequence from the state of Washington in Spring 2010 has recently been profiled with similar zoonotic influences.



* Rare, not Random.

Please visit GeneWurx.com for insight into the latest published studies.

225A Appears In Madrid Spain Pandemic Influenza

The very effective contributors to The NIAID Influenza Genome Sequencing Consortium deposited a contemporaneous set of sequences at GISAID this week covering a significant number of data points over geography and emerging trends in pandemic RnR * polymorphism.

225A is found for the first time on an unmixed trace in this pandemic on Madrid296_2009_11_17, having previously been discussed on an unusual sequence from the Ukraine. The HA of UkrKyiv377_2009_11_07_xL only revised at two protein positions, the syn413K for cross-linkage and amino residue 225 (rvT coding up to 6 values).  These multiple, mixed traces at the first and second nucleotides of the codon represent the only documented prior instance of potential 225A in this pandemic reservoir.

Additionally, a narrow slice of the data in this directed review further suggests a peculiar behaviour that our team has postulated in the past 3 months within this present pandemic reservoir (PF11). Spain and Greece provide an active working ground for this particular genetic acquisition cycle behaviour.

Notice that the reservoir appears to individually conserve polymorphisms on strains having few revisions (generalised / consensus strains) from time to time, even after the given polymorphism has appeared on a significant count of sequences having numerous polymorphisms.  This apparent reversal of progressive genetic acquisition may have purpose. 

Preservation behaviour for future recombination using / by creating a type of "universal donor" template is suggested.  This step-wise method of storing an important piece of data for later usage in a labile format is not entirely novel in biology.  Ease of access / transfer / adoption may be increased as a function of the homology / identity of the two interchanging viral strains.  “Mix and match” becomes more organised and discrete in this scenario. Ergo, a generalised virus with only a single revision or a minimal revision count may be more successful as an overlay for material transfer.

Note that Madrid300_2009_11_30 carries only the 373H.  Similarly, notice that syn372Q (in a similar domain) is found on Russian fatality sequences that have no change other than the cross-linkage syn413K.  The syn372Q is also found upon a 225G and a fatal 225N case in the same area, Ulyanovsk. 

Is a similar function within the same sequence area (372, 373) being preserved in Spain, Greece, Portugal, Belgium, Germany and Australia using the 373H?

Is the Madrid296_2009_11_17 pairing of 225A (in its first recorded occurrence) with 373H another of the reservoir's conservation storage templates that will be referenced for later antigenic escape?

. . . . Athens357_2009_12_26 (
. . . . . . . . 1S,
. . . . . . . . 92M,
. . . . . . . . syn181G [H9N2],
. . . . . . . . . . . . . . . [Lisboa2_2010_01_04,
. . . . . . . . . . . . . . . Rome632_2009_11_23, et al],
. . . . . . . . 373H [Perth500_2010_02_17 with 165N,
. . . . . . . . . . . . . Tessenderlo191_2009_12_15
. . . . . . . . . . . . . . . . . . . . . with 165R, syn181G,
. . . . . . . . . . . . . Lisboa171_2009_12_02])

. . . . Athens358_2009_12_26 (
. . . . . . . . syn179L (CTt) [TexasJMS404_2010_01_08 (tTA)
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . with syn66G,
. . . . . . . . . . . . . . . . . . . . . Netherlands2143_2009_11_16 (tTA),
. . . . . . . . . . . . . . . . . . . . . Odense143_2009_11_11 (tTA)
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . with 48K, 269V,
. . . . . . . . . . . . . . . . . . . . . UkrPoltava746_2009_11_09_f (tTA),
. . . . . . . . . . . . . . . . . . . . . UkrCherkasy745_2009_11_01_f (tTA),
. . . . . . . . . . . . . . . . . . . . . Antwerp221_2009_10_28 (tTA)
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . with 259T,
. . . . . . . . . . . . . . . . . . . . . JapanAF2244_2009_10_14 (CTg),
. . . . . . . . . . . . . . . . . . . . . ColoradoAF2139_2009_10_06 (CTg)
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . with syn311K, 324I,
. . . . . . . . . . . . . . . . . . . . . NY4981_2009_10_05 (CTg)
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . with syn311K, 324I,
. . . . . . . . . . . . . . . . . . . . . ThailandPMKD0030_2009_09_25 (tTA),
. . . . . . . . . . . . . . . . . . . . . WiscS0143_2009_06_02 (CTg),
. . . . . . . . . . . . . . . . . . . . . WiscD1725_2009_06_01 (CTg),
. . . . . . . . . . . . . . . . . . . . . WiscD0117_2009_05_28 (CTg)]
. . . . . . . . 373H [Perth500_2010_02_17 with 165N,
. . . . . . . . . . . . . Tessenderlo191_2009_12_15
. . . . . . . . . . . . . . . . . . . . . with 165R, syn181G,
. . . . . . . . . . . . . Lisboa171_2009_12_02])

. . . . Munich364_2009_12_04 (
. . . . . . . . #1V,
. . . . . . . . syn181G [H9N2],
. . . . . . . . . . . . . . . [Lisboa2_2010_01_04,
. . . . . . . . . . . . . . . Rome632_2009_11_23, et al],
. . . . . . . . 373H [Perth500_2010_02_17 with 165N,
. . . . . . . . . . . . . Tessenderlo191_2009_12_15
. . . . . . . . . . . . . . . . . . . . . with 165R, syn181G,
. . . . . . . . . . . . . Lisboa171_2009_12_02],
. . . . . . . . syn396V,
. . . . . . . . syn477T)

. . . . Munich363_2009_11_30 (
. . . . . . . . 165R [Tessenderlo191_2009_12_15
. . . . . . . . . . . . . . . . . . with syn181G],
. . . . . . . . syn181G [H9N2],
. . . . . . . . . . . . . . . [Lisboa2_2010_01_04,
. . . . . . . . . . . . . . . Rome632_2009_11_23, et al],
. . . . . . . . 373H [Perth500_2010_02_17 with 165N,
. . . . . . . . . . . . . Tessenderlo191_2009_12_15
. . . . . . . . . . . . . . . . . . . . . with 165R, syn181G,
. . . . . . . . . . . . . Lisboa171_2009_12_02],
. . . . . . . . syn488D,
. . . . . . . . syn514Y)

. . . . Madrid300_2009_11_30 (
. . . . . . . . 373H [Perth500_2010_02_17 with 165N,
. . . . . . . . . . . . . Tessenderlo191_2009_12_15
. . . . . . . . . . . . . . . . . . . . . with 165R, syn181G,
. . . . . . . . . . . . . Lisboa171_2009_12_02])

. . . . Madrid299_2009_11_26 (
. . . . . . . . 128I,
. . . . . . . . 235I,
. . . . . . . . 373H [Perth500_2010_02_17 with 165N,
. . . . . . . . . . . . . Tessenderlo191_2009_12_15
. . . . . . . . . . . . . . . . . . . . . with 165R, syn181G,
. . . . . . . . . . . . . Lisboa171_2009_12_02],
. . . . . . . . syn453R)

. . . . Madrid298_2009_11_24 (
. . . . . . . . 0I [H9N2],
. . . . . . . . .  [Lisboa2_2010_01_04,
. . . . . . . . . Latvia12_649_2009_12_09,
. . . . . . . . . NY7426_2009_12_08,
. . . . . . . . . Tambov_FOS_2009_12_01_xL,
. . . . . . . . . Slovenia5555_2009_12,
. . . . . . . . . Slovenia5559_2009_12,
. . . . . . . . . Ivanovo_RNA_2009_11_24_xL_f with 225G, 225N,
. . . . . . . . . Stockholm107_2009_11_24,
. . . . . . . . . GD_Yuncheng51_2009_11_19,
. . . . . . . . . Guangdong1271_2009_11_19,
. . . . . . . . . Austria528378_2009_11_19,
. . . . . . . . . Latvia11_1133_2009_11_16,
. . . . . . . . . Latvia11_1133M_2009_11_16,
. . . . . . . . . Latvia11_575_2009_11_11,
. . . . . . . . . England94600039_2009_10_27,
. . . . . . . . . SwedenUmea8_2009_10_07,
. . . . . . . . . Managua2323_02_2009_08_18,
. . . . . . . . . Utah20_C2_2_2009_07_25_VxX with 159D, 227G,
. . . . . . . . . Texas42163291_2009_06_16,
. . . . . . . . . Marseille3416_2009],
. . . . . . . . syn181G [H9N2],
. . . . . . . . . . . . . . . [Lisboa2_2010_01_04,
. . . . . . . . . . . . . . . Rome632_2009_11_23, et al],
. . . . . . . . 233H [NC Duke TmX Fatality cluster,
. . . . . . . . . . . . Rome632_2009_11_23 with syn181G],
. . . . . . . . 373H [Perth500_2010_02_17 with 165N,
. . . . . . . . . . . . . Tessenderlo191_2009_12_15
. . . . . . . . . . . . . . . . . . . . . with 165R, syn181G,
. . . . . . . . . . . . . Lisboa171_2009_12_02],
. . . . . . . . 504N [England735_2009_10,
. . . . . . . . . . . . England1023_2009_09,
. . . . . . . . . . . . England (11),
. . . . . . . . . . . . Scotland94420091_2009_08_11])

. . . . Madrid296_2009_11_17 (
. . . . . . . . 225A [Unique to PF11],
. . . . . . . . 373H [Perth500_2010_02_17 with 165N,
. . . . . . . . . . . . . Tessenderlo191_2009_12_15
. . . . . . . . . . . . . . . . . . . . . with 165R, syn181G,
. . . . . . . . . . . . . Lisboa171_2009_12_02])

Please refer to additional studies for further genetic analyses, including the survey on amino acid revisions potentially related to Vaccine Escape.  A hyper-morphic, TamiFlu-resistant sequence from the state of Washington in Spring 2010 has recently been profiled with similar zoonotic influences.


* Rare, not Random.

Please visit GeneWurx.com for insight into the latest published studies.